Department of Integrative Biology
Graduate Thesis Defense.

Congratulations to Sarah Louie M.S. Student in Dr. Brad Stith’s lab on her successful thesis defense!  Sarah has accepted an offer from Oregon State’s Biochemistry and Biophysics PhD program and is “very excited” about the next chapter in her educational journey.  We wish you the very best Sarah!

Sarah Louie
M.S. Degree Candidate
CU Denver Department of Integrative Biology
Dr. Brad Stith’s Lab

When: April 18, 2022
Where: Science Building 4127, at 2:00.

Sperm, phosphatidic acid, and calcium ionophore induce Xenopus egg Src cycling and tyrosine phosphatase activity. 

Src tyrosine kinase holds an essential role in the regulation of the fertilization signaling pathway in Xenopus laevis. This work describes the cycling of two regulatory phosphorylation sites of Src that can be induced in eggs through insemination, phosphatidic acid, or calcium ionophore addition. Activation of tyrosine phosphatases also increases in eggs with the addition of these three activators. This suggests an undescribed role of tyrosine phosphatases in fertilization and a potential negative feedback loop between Src and PTPases, which is examined with the use of PTP Inhibitor I.