We work with a variety of experimental procedures from southern blotting to mammalian cell culture and have posted here what works for us. Feel free to download and use any of our protocols and don’t hesitate to contact us with any questions.
Transfection of mouse embryonic stem cells with PEI: see our publication in Experimental Cell Research
Methylation Analysis:
- gDNA Isolation for Bisulfite Sequencing
- Bisulfite Conversion of Genomic DNA – we have used both the Invitrogen MethylCodeTMBisulfite Conversion Kit and the Zymo Research EZ DNA MethylationTM Kit with great success
- Primer Design Strategy for Bisulfite Sequencing
- Sequencing of Bisulfite Converted DNA – coming soon
DNA cloning:
- Invitrogen Gateway cloning– Also see Invitrogen’s website for more information
- Mutagenesis
Mouse work:
Recombineering for Conditional Knockout Mice
Protein: