Amanda Charlesworth photo
Ph.D. • Associate Professor • Interim Department Chair
Department of Integrative Biology

Mailing Address:
Department of Integrative Biology
Campus Box 171
P.O. Box 173364
Denver, CO 80217-3364

Physical Location:
1150 12th Street
SI 4106
Denver, CO 80204

Office Hours:  Mon 2 – 3 pm, Wed 5 – 6 pm, or by appointment.

Chair Office Hours:  BY APPOINTMENT

Expertise Areas:
Molecular developmental biology. Regulation of gene expression by RNA translational control and its role in cell cycle progression during oocyte maturation, maternal to embryo transition, and early development.

B.Sc. Biochemistry, University of Kent at Canterbury, UK  (1992)
Ph.D. Cell Biology, University College London, UK (1996)

I have a background in signal transduction and cell cycle regulation in the fields of neurobiology, cancer biology and developmental biology, using biochemical, molecular biology and cell biology approaches.  These experiences were acquired in large pharmaceutical companies, medical research institutes, medical schools and undergraduate universities.

My current research focuses on gene expression in early development. After fertilization the male genome is not used immediately.  Instead, important developmental process are controlled by mRNAs that were pre-loaded into the egg by the female.  Important questions that we don't know the answers to are how are these mRNAs used at the right time, in the right place.

The mRNAs that are donated by the female contain instructions that control the time of their activation. My lab is identifying and characterising these instructions.  We are also identifying and characterising the factors that decode these instructions.

Student researchers gain experience and expertise in cell and molecular biology methods including protein analysis, cloning, cell based luciferase reporter assays, and RT-PCR-based RNA analysis.

15 out of 27

2017    Cook, J. M., and Charlesworth, A. (2017) Insertion of inter-domain linkers improves expression and bioactivity of Zygote arrest (Zar) fusion proteins. Prot. Eng. Des. Sel. doi: 10.1093/protein/gzx002 Pubmed

2013    Yamamoto, T., Cook, J. M., Kotter, C. V., Khat, T., Silva, K. D., Ferreyros, M, Holt, J. W., Knight, J. D., and Charlesworth, A.  (2013)  Zar1 represses translation in Xenopus oocytes and binds to the TCS in maternal mRNAs with different characteristics than Zar2.  Biochimica et biophysica acta, 1829: 1034-1046.  PubMed

2013    Charlesworth, A., Meijer, H.A., and de Moor, C.H.  (2013)  Specificity factors in cytoplasmic polyadenylation.  (2013) WIREs RNA, 4: 437-461  (Invited Review)  PubMed

2012    Charlesworth A, Yamamoto TM, Cook JM, Silva KD, Kotter CV, Carter GS, Holt JW, Lavender HF, MacNicol AM, Wang YY, Wilczynska A. (2012) Xenopus laevis Zygote arrest 2 (zar2) encodes a zinc finger RNA-binding protein that binds to the Translational Control Sequence in the maternal Wee1 mRNA and regulates translation.  Dev. Bio., 369:177-190  PubMed

2008    Wang, Y., Charlesworth, A., Byrd, S.M., Gregerson, R., MacNicol, M.C., MacNicol, A.M.  (2008) A novel mRNA 3' untranslated region translational control sequence regulates Xenopus Wee1 mRNA translation.  Dev. Bio., 317: 454-466  PubMed

2006    Charlesworth, A., Wilczynska, A., Thampi, P., Cox, L.L. and MacNicol, A.M.  (2006) Musashi regulates the temporal order of mRNA translation during Xenopus oocyte maturation.  EMBO J.,  25: 2792-2801  PubMed

2004    Charlesworth A., Cox L. L. and MacNicol A.M.  (2004) Cytoplasmic Polyadenylation Element (CPE)- and CPE-binding Protein (CPEB)-independent Mechanisms Regulate Early Class Maternal mRNA Translational Activation in Xenopus Oocytes.  J. Biol. Chem. 279: 17650-17659  PubMed

2002    Charlesworth A., Ridge J.A., King L.A., MacNicol M.C. and MacNicol A.M.  (2002) A novel regulatory element determines the timing of Mos mRNA translation during Xenopus oocyte maturation.  EMBO J., 21: 2798-2806.  PubMed

2001    Welk J.F., Charlesworth A. and MacNicol A.M.  (2001) Identification and characterization of a human cytoplasmic polyadenylation element binding protein. Gene, 263: 113-120.  PubMed

2000    Charlesworth A., Welk J. and MacNicol A.M.  (2000) The temporal control of Wee1 mRNA translation during Xenopus oocyte maturation is regulated by cytoplasmic polyadenylation elements within the 3’ untranslated region.  Dev. Bio., 227: 706-719.  PubMed

1999    Howard E.L., Charlesworth A., Welk J. and MacNicol A.M.  (1999) The MAP kinase signaling pathway stimulates Mos mRNA cytoplasmic polyadenylation during Xenopus oocyte maturation.  Mol. Cell. Biol., 19: 1990-1999.  PubMed

1997    Charlesworth A. and Rozengurt E.  (1997) Bombesin and neuromedin B stimulate the activation of p42mapk and p74raf-1 via a PKC-independent pathway in Rat-1 cells.  Oncogene, 14: 2323 – 2329.  PubMed

1996    Charlesworth A., Broad S. and Rozengurt E.  (1996) The bombesin/GRP receptor transfected into Rat-1 fibroblasts couples to phospholipase C activation, tyrosine phosphorylation of p125FAK and paxillin and cell proliferation.  Oncogene, 12: 1337-1345.  PubMed

1994    Charlesworth A. and Rozengurt E.  (1994) Thapsigargin and di-tert-butylhydroquinone induce synergistic stimulation of DNA synthesis with phorbol ester and bombesin in Swiss 3T3 cells.  J. Biol. Chem., 269: 32528 – 32535.  PubMed

1992    McAllister G., Charlesworth A., Snodin C., Beer M.S., Noble A.J., Middlemiss D.N., Iversen L.L. and Whiting P.  (1992) Molecular cloning of a serotonin receptor from human brain (5HT1E): A fifth 5HT1-like subtype.  Proc. Natl. Acad. Sci., USA 89: 5517- 5521.  PubMed

BIOL 3611 General Cell Biology
BIOL 4068/5068 Cell Cycle
BIOL 4634/5634 Biology of Cancer
BIOL 4050/5050 Topics in Developmental Biology
BIOL 5705 Principles of Research
BIOL 4990/6655 Seminar