There has been an explosion of techniques that allow for the interrogation of cellular signaling at its most basic levels. As these techniques have matured, we have begun to realize that many biological processes, such as gene expression, are well described by stochastic physics at their core level. However, we are still lacking both experimental and theoretical tools to connect these basic understanding of molecular biology to larger-scale issue, such as genetic risk factors for disease.
Our laboratory is specifically interested in measuring the genetic response of key regulatory genes in primary mammalian cells lines to better understand the bio-molecular basis for disease. We are currently focused on the role that endothelial progenitor cells play in the developing human lung, particularly with respect to oxidative stress.
Previously, we demonstrated the first direct imaging detection of novel small RNA that regulate pathogensis in Yersinia Pestis (the caustive agent of the bubonic plague) and other bacteria. This work was highlighted in a Los Alamos National Laboratory Science Feature Story.
Our research combines cutting edge techniques from single-molecule biophysics, quantitative imaging, GPU-based computation, molecular biochemistry, and genetic regulation to create a statistical understanding of genetic expression that complements our existing biological knowledge. This research draws from physics, biology, chemistry, and mathematics.
If this research engages you, please contact Doug Shepherd for more information.
We currently have several open positions available.